MORPHOMETRIC ANALYSIS OF STRUCTURAL CHANGES IN ENDOTHELIAL CELLS OF ARTERIES AND VEINS OF TESTES IN THE CONDITIONS AT POSTRESECTION PORTAL HYPERTENSION

  • S. O. Konovalenko Ternopil National Medical University named by I.Ya. Horbachevsky, Department of Operative Surgery and Clinical Anatomy,Ternopil, Ukraine https://orcid.org/0000-0002-3478-462X
Ключові слова: postresection portal hypertension, testes, endotheliocytes, arteries, veins

Анотація

Removal of large volumes of liver can lead to postresection portal hypertension, venous stasis in the organs of the portal system and structural and functional changes in them. Morphological changes in endotheliocytes of arteries and veins of testes in postresection portal hypertension are insufficiently studied.The purpose of the study: to perform morphometric analysis of structural changes in endotheliocytes of arteries and veins of testes in the conditions of postresection portal hypertension and multiorgan failure.Materials and methods. The testes of 50 white male rats, which were divided into 3 groups, were morphometrically examined. The 1st group consisted of 15 intact animals, 2 - 25 rats with postresection portal hypertension, 3 - 10 animals with postresection portal hypertension and multiorgan failure. Postresection portal hypertension was simulated by removing the left and right lateral lobes of the liver. Euthanasia of rats was performed by bloodletting under conditions of thiopental anesthesia one month after the start of the experiment. Testes were used to make micropreparations, which morphometrically determined the height of endotheliocytes  of arteries and veins, the diameter of their nuclei, nuclear-cytoplasmic ratios in these cells, the relative volumes of damaged endotheliocytes. Quantitative morphological parameters were processed statistically.Results. It was found that in portal hypertension the relative volume of damaged endotheliocytes in the arteries of the left testis increased by 2.04 times, in the right - 1.87 times (p˂0.001), and in the veins, respectively - 17 times and 15, 1 times (p <0.001). In the conditions of postresection portal hypertension and multiorgan insufficiency, the relative volume of damaged endotheliocytes in the arteries of the left testis increased by 11, the right by 9.2 times (p˂0.001), and in the veins, respectively - by 20.8 (p˂0.001) and 17.5 times (p˂0.001).Optical on histological preparations of testes showed scattering and dilation of venous vessels, which was complicated by hypoxia, dystrophy, necrobiosis of cells and tissues of the studied organ, and in the longterm to infiltrative and sclerotic processes. Venous vessels of the microhemocirculatory tract are unevenly dilated, tortuous, full-blooded, with numerous varicose veins and sacculations. Stagnation, thrombosis, diapedetic hemorrhages, plasmorrhagia of the walls of venous vessels and paravasal tissues were detected in these vessels. Elastic structures in vessels with the phenomena of multiplication, fragmentation and destruction. Endotheliocytes with signs of edema, dystrophically and necrobiotically altered, sometimes desquamated. Fociofcellularin filtration and sclerosis observed in  the stroma of the testes. Sealed structural changes dominated in the left testis and in the conditions of postresection portal hypertension with multiorgan failure. The predominance of morphological and morphometric changes in the vessels of the left testis can be explained by the peculiarities of venous outflow from this organ.Conclusions. The obtained data indicate that postresection portal hypertension leads to violations of cellular structural homeostasis, an increase in the relative volumes of damaged endotheliocytes in the vascular bed of the testes. The identified processes dominate in the venous bed of the left testis and in the combination of postresection portal hypertension with multiorgan failure.

Опубліковано
2020-07-05
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